Science Spotlight: Bending Light with a New Kind of Microscope
Diagnosing Diseases With Origami Microscopes
X-ray Microscope: Seeing Cells in 3-D
50 Years Later, Still Plenty of Room at the Bottom
The World's Most Powerful Microscope
Producer's Notes: World's Most Powerful Microscope
Sponsored
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Stream or download it on \u003ca href=\"http://www.pbslearningmedia.org/resource/31a22583-dcee-420a-9f8f-8966c86dbf8e/science-spotlight-bending-light-with-a-new-kind-of-microscope/\"> PBS LearningMedia\u003c/a>.\u003c/em>\u003c/p>\n\u003cp>\u003cem>Article by Lauren Farrar\u003c/em>\u003c/p>\n\u003cp>Manu Prakash, a bioengineer at Stanford University, has created a fully functional microscope out of waterproof paper that uses teeny-tiny lenses to magnify objects. He calls it a Foldscope. The different parts of the microscope are printed on paper, which the user punches out and folds together. The Foldscope requires no power outlets and works with standard microscope slides.\u003c/p>\n\u003cfigure id=\"attachment_74155\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/04/Foldscope_CU_small_white.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/04/Foldscope_CU_small_white-300x169.jpg\" alt=\"the Foldscope\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-74155\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">A close up of the Foldscope.\u003c/figcaption>\u003c/figure>\n\u003cp>Microscopes are important tools that allow people to view objects that are so small that you wouldn’t ordinarily be able to see them, like bacteria or blood cells. From healthcare workers using microscopes to look at blood samples to diagnose diseases, to forensic scientists using them to study evidence from crime scenes, many different kinds of microscopes have been developed for a variety of purposes.\u003c/p>\n\u003cp>The Foldscope operates a lot like a traditional microscope in that it uses lenses to bend light in order to make tiny images appear larger. Light travels in waves and when it passes through media with a different density than air, such as water, the light changes direction, or bends. This is why a pencil looks bent when it is halfway submerged in a glass of water. This bending of light is known as refraction. Curved objects, such as lenses also bend light. How the light bends depends on how curved the lens is and the material it's made out of. Convex lenses, which are thicker in the middle and curve outward, can be used to magnify objects.\u003c/p>\n\u003cfigure id=\"attachment_74063\" class=\"wp-caption alignleft\" style=\"max-width: 640px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/05/Lense_schematic_small.jpg\">\u003cimg class=\"size-large wp-image-74063\" src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/05/Lense_schematic_small-640x360.jpg\" alt=\"schematic of a convex lens\" width=\"640\" height=\"360\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">A convex lens can bend light and make an object appear larger by creating a larger virtual image that your eye can see.\u003c/figcaption>\u003c/figure>\n\u003cp>A magnifying glass is the simplest kind of microscope -- a convex lens with a handle attached. How much an object is magnified depends on several factors, including how curved the lens is, the distance between the lens and the object, and the distance between the lens and the user’s eye. The more curved the lens is, the higher the magnification it offers. Magnifying glasses are limited in how much they can magnify an object; usually, they can make objects look around 2-10 times larger than they actually are. More advanced microscopes combine multiple lenses to bend light in different ways in order to magnify objects even more.\u003c/p>\n\u003cp>[ad fullwidth]\u003c/p>\n\u003cp>Foldscopes use salt-grain sized lenses that are highly curved. The extreme curvature of these lenses allow for high magnification. For this reason, Foldscopes don’t require long tubes of stacked lenses to magnify objects, like other microscopes do. This is why the Foldscope looks flat compared to larger, more traditional microscopes. Even though they are made out of waterproof paper and tiny lenses, Foldscopes can make objects appear 2,000 times larger than they actually are. This makes it possible to see really small things, like cells and bacteria.\u003c/p>\n\u003cp>This video is part of our \u003ca href=\"http://foldscope.woop.ie/\"> Engineering Is Diagnosing Diseases with Origami Microscopes e-book\u003c/a>. The e-book explores the science and engineering principles behind Manu Prakash’s Foldscopes project, and includes videos, interactives and media making opportunities. You can find our other e-books at \u003ca href=\"http://blogs.kqed.org/education/e-books/\">kqed.org/ebooks\u003c/a>.\u003c/p>\n\u003cp>\u003c/p>\n\u003cp>For more information about microscopes and lenses check out these resources from \u003ca href=\"http://www.explainthatstuff.com/\" target=\"_blank\">Explain that Stuff\u003c/a>:\u003c/p>\n\u003cul>\n\u003cli>\u003ca href=\"http://www.explainthatstuff.com/microscopes.html\">How Microscopes Work\u003c/a>\u003c/li>\n\u003cli>\u003ca href=\"http://www.explainthatstuff.com/lenses.html\">Lenses\u003c/a>\u003c/li>\n\u003c/ul>\n\n","blocks":[],"excerpt":"Learn how microscopes work by using lenses to bend light to magnify objects.","status":"publish","parent":0,"modified":1442618052,"stats":{"hasAudio":false,"hasVideo":false,"hasChartOrMap":false,"iframeSrcs":[],"hasGoogleForm":false,"hasGallery":false,"hasHearkenModule":false,"hasPolis":false,"paragraphCount":11,"wordCount":562},"headData":{"title":"Science Spotlight: Bending Light with a New Kind of Microscope | KQED","description":"Learn how microscopes work by using lenses to bend light to magnify objects.","ogTitle":"","ogDescription":"","ogImgId":"","twTitle":"","twDescription":"","twImgId":""},"disqusIdentifier":"74061 http://science.kqed.org/quest/?p=74061","disqusUrl":"https://ww2.kqed.org/quest/2015/05/07/science-spotlight-bending-light-with-a-new-kind-of-microscope/","disqusTitle":"Science Spotlight: Bending Light with a New Kind of Microscope","videoEmbed":"http://www.youtube.com/watch?v=WwLBw13NKNU","source":"Physics","sourceUrl":"https://ww2.kqed.org/quest/category/physics/","path":"/quest/74061/science-spotlight-bending-light-with-a-new-kind-of-microscope","audioTrackLength":null,"parsedContent":[{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003cp>\u003cem>Trouble viewing the video? Stream or download it on \u003ca href=\"http://www.pbslearningmedia.org/resource/31a22583-dcee-420a-9f8f-8966c86dbf8e/science-spotlight-bending-light-with-a-new-kind-of-microscope/\"> PBS LearningMedia\u003c/a>.\u003c/em>\u003c/p>\n\u003cp>\u003cem>Article by Lauren Farrar\u003c/em>\u003c/p>\n\u003cp>Manu Prakash, a bioengineer at Stanford University, has created a fully functional microscope out of waterproof paper that uses teeny-tiny lenses to magnify objects. He calls it a Foldscope. The different parts of the microscope are printed on paper, which the user punches out and folds together. The Foldscope requires no power outlets and works with standard microscope slides.\u003c/p>\n\u003cfigure id=\"attachment_74155\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/04/Foldscope_CU_small_white.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/04/Foldscope_CU_small_white-300x169.jpg\" alt=\"the Foldscope\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-74155\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">A close up of the Foldscope.\u003c/figcaption>\u003c/figure>\n\u003cp>Microscopes are important tools that allow people to view objects that are so small that you wouldn’t ordinarily be able to see them, like bacteria or blood cells. From healthcare workers using microscopes to look at blood samples to diagnose diseases, to forensic scientists using them to study evidence from crime scenes, many different kinds of microscopes have been developed for a variety of purposes.\u003c/p>\n\u003cp>The Foldscope operates a lot like a traditional microscope in that it uses lenses to bend light in order to make tiny images appear larger. Light travels in waves and when it passes through media with a different density than air, such as water, the light changes direction, or bends. This is why a pencil looks bent when it is halfway submerged in a glass of water. This bending of light is known as refraction. Curved objects, such as lenses also bend light. How the light bends depends on how curved the lens is and the material it's made out of. Convex lenses, which are thicker in the middle and curve outward, can be used to magnify objects.\u003c/p>\n\u003cfigure id=\"attachment_74063\" class=\"wp-caption alignleft\" style=\"max-width: 640px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/05/Lense_schematic_small.jpg\">\u003cimg class=\"size-large wp-image-74063\" src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/05/Lense_schematic_small-640x360.jpg\" alt=\"schematic of a convex lens\" width=\"640\" height=\"360\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">A convex lens can bend light and make an object appear larger by creating a larger virtual image that your eye can see.\u003c/figcaption>\u003c/figure>\n\u003cp>A magnifying glass is the simplest kind of microscope -- a convex lens with a handle attached. How much an object is magnified depends on several factors, including how curved the lens is, the distance between the lens and the object, and the distance between the lens and the user’s eye. The more curved the lens is, the higher the magnification it offers. Magnifying glasses are limited in how much they can magnify an object; usually, they can make objects look around 2-10 times larger than they actually are. More advanced microscopes combine multiple lenses to bend light in different ways in order to magnify objects even more.\u003c/p>\n\u003cp>\u003c/p>\u003c/div>","attributes":{"named":{},"numeric":[]}},{"type":"component","content":"","name":"ad","attributes":{"named":{"label":"fullwidth"},"numeric":["fullwidth"]}},{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003c/p>\n\u003cp>Foldscopes use salt-grain sized lenses that are highly curved. The extreme curvature of these lenses allow for high magnification. For this reason, Foldscopes don’t require long tubes of stacked lenses to magnify objects, like other microscopes do. This is why the Foldscope looks flat compared to larger, more traditional microscopes. Even though they are made out of waterproof paper and tiny lenses, Foldscopes can make objects appear 2,000 times larger than they actually are. This makes it possible to see really small things, like cells and bacteria.\u003c/p>\n\u003cp>This video is part of our \u003ca href=\"http://foldscope.woop.ie/\"> Engineering Is Diagnosing Diseases with Origami Microscopes e-book\u003c/a>. The e-book explores the science and engineering principles behind Manu Prakash’s Foldscopes project, and includes videos, interactives and media making opportunities. You can find our other e-books at \u003ca href=\"http://blogs.kqed.org/education/e-books/\">kqed.org/ebooks\u003c/a>.\u003c/p>\n\u003cp>\u003c/p>\n\u003cp>For more information about microscopes and lenses check out these resources from \u003ca href=\"http://www.explainthatstuff.com/\" target=\"_blank\">Explain that Stuff\u003c/a>:\u003c/p>\n\u003cul>\n\u003cli>\u003ca href=\"http://www.explainthatstuff.com/microscopes.html\">How Microscopes Work\u003c/a>\u003c/li>\n\u003cli>\u003ca href=\"http://www.explainthatstuff.com/lenses.html\">Lenses\u003c/a>\u003c/li>\n\u003c/ul>\n\n\u003c/div>\u003c/p>","attributes":{"named":{},"numeric":[]}}],"link":"/quest/74061/science-spotlight-bending-light-with-a-new-kind-of-microscope","authors":["10423"],"categories":["quest_8","quest_16"],"tags":["quest_12946","quest_13162","quest_12269","quest_3351","quest_1646","quest_13167","quest_13161","quest_13169","quest_1815","quest_13166","quest_1816","quest_2083","quest_2141","quest_2349","quest_13168","quest_13142"],"collections":["quest_13175"],"featImg":"quest_74077","label":"source_quest_74061"},"quest_74045":{"type":"posts","id":"quest_74045","meta":{"index":"posts_1591205157","site":"quest","id":"74045","score":null,"sort":[1430437639000]},"guestAuthors":[],"slug":"diagnosing-diseases-with-origami-microscopes-engineering-is","title":"Diagnosing Diseases With Origami Microscopes","publishDate":1430437639,"format":"video","headTitle":"QUEST | KQED Science","labelTerm":{},"content":"\u003cp>\u003cem>Trouble viewing the video? Stream or download it on \u003ca href=\"http://www.pbslearningmedia.org/resource/727416dd-b83b-4cfb-abbc-27ec2651629b/diagnosing-diseases-with-origami-microscopes/\"> PBS LearningMedia\u003c/a>.\u003c/em>\u003c/p>\n\u003cp>\u003cem>Article by Lauren Farrar\u003c/em>\u003c/p>\n\u003cp>“What if you could drop microscopes literally around the world from an airplane?” Manu Prakash, a professor of bioengineering at Stanford University would often joke with his team.\u003c/p>\n\u003cp>This musing actually heavily influenced the design of their new microscope, a paper origami microscope. They call it a Foldscope. The microscope is printed on waterproof paper. The user punches out the pieces and folds them together to create a fully functional microscope. It works with standard microscope slides and requires no batteries or electricity to operate. You simply hold the Foldscope up to a light source (like the sun) and look through the salt grain-sized lens to view the sample on the slide. The high curvature of the tiny lenses used in the Foldscope allows small objects to be highly magnified. This little invention costs less than a dollar to produce and could have major implications for global health and for science education.\u003c/p>\n\u003cfigure id=\"attachment_74155\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/04/Foldscope_CU_small_white.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/04/Foldscope_CU_small_white-300x169.jpg\" alt=\"the Foldscope\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-74155\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">A close up of the Foldscope.\u003c/figcaption>\u003c/figure>\n\u003cp>Prakash grew up in small towns in India where access to healthcare and diagnostic tools, like microscopes, weren’t always easy to come by. He has traveled extensively throughout developing countries and has seen firsthand how diseases like malaria, African sleeping sickness and schistosomiasis can go untreated or are mistreated because patients are not properly diagnosed. Diagnosis of diseases like these usually require a microscope to identify the presence of a particular parasite so that the proper treatment can be administered. But remote or resource poor areas don’t always have access to these heavy, expensive microscopes used in other parts of the world. Standard microscopes are also difficult to transport when doctors travel from village to village to diagnose and treat patients. And even when remote places do have microscopes, there is little support to maintain them or parts available to fix them if they break.\u003c/p>\n\u003cp>[ad fullwidth]\u003c/p>\n\u003cp>“Humidity and tropics are terrible for optics… Most of the time when we’re in the field there is fungus growing on the lenses… There were times when we were imaging there, we saw an insect colony come out of the microscope,” Prakash describes some of the challenges of doing field microscopy in remote tropical areas.\u003c/p>\n\u003cp>Prakash was determined to build a cheap, robust, easy-to-use and easy-to-fix microscope that required no power and worked with traditional microscope slides. After prototyping with simple supplies like paper, tape and scissors, he and his team came up with a design that worked. The base level requires no external power source and relies on the sun as the primary light source. The team has since developed multiple versions that allow users to do different kinds of microscopy. For example, there are versions with external light modules, versions of various magnifications, versions that allow for fluorescence microscopy, versions that allow for bright field microscopy, versions that allow for dark field microscopy, and versions that allow the user to project the image on a wall. You can even attach a smartphone to the microscope in order to record images.\u003c/p>\n\u003cp>The Foldscopes are currently undergoing validation studies and clinical trials to be approved as diagnostic tools for diseases including malaria, African sleeping sickness and schistosomiasis.\u003c/p>\n\u003cp>In addition to their potential value for global health, Foldscopes have a strong educational appeal. Prakash wanted to get these microscopes in the hands of children everywhere, so he started the \u003ca href=\"http://www.foldscope.com/\"> Ten Thousand Microscope project\u003c/a>. He wanted to create a network of learners asking questions, so he put a call out for people to submit an idea for an experiment that they would like to do with the Foldscope and then report back on their findings. This idea was so well received that he has shipped approximately 50,000 microscopes to people all around the world. Some of them are already reporting their findings on his \u003ca href=\"http://microcosmos.foldscope.com/\"> website\u003c/a>. He hopes that this project will inspire curiosity and scientific thinking.\u003c/p>\n\u003cp>“Scientific tools need to become common day-to-day objects if we want people to think scientifically,” he says.\u003c/p>\n\u003cp>While Foldscopes used for diagnostic testing come pre-folded to ensure high quality control, the ones sent out for educational purposes through the Ten Thousand Microscope Project are folded and built by the users. He hopes that school children, hackers, and tinkerers all over the world will build on and add to the microscopes. While Prakash isn’t quite dropping these Foldscopes from airplanes, he’s certainly getting them into the hands of children all around the world.\u003c/p>\n\u003cp>\u003c/p>\n\u003cp>This video is part of our \u003cstrong>\u003ca href=\"http://foldscope.woop.ie/\" target=\"_blank\">Engineering Is: Diagnosing Diseases with Origami Microscopes\u003c/a>\u003c/strong> e-book. The e-book explores the science and engineering principles behind Manu Prakash’s Foldscopes project, and includes videos, interactives and media making opportunities. You can find our other e-books at \u003ca href=\"http://blogs.kqed.org/education/e-books/\"> kqed.org/ebooks\u003c/a>.\u003c/p>\n\n","blocks":[],"excerpt":"Manu Prakash has designed the Foldscope, an origami paper microscope that costs less than a dollar.","status":"publish","parent":0,"modified":1450483326,"stats":{"hasAudio":false,"hasVideo":false,"hasChartOrMap":false,"iframeSrcs":[],"hasGoogleForm":false,"hasGallery":false,"hasHearkenModule":false,"hasPolis":false,"paragraphCount":14,"wordCount":823},"headData":{"title":"Diagnosing Diseases With Origami Microscopes | KQED","description":"Manu Prakash has designed the Foldscope, an origami paper microscope that costs less than a dollar.","ogTitle":"","ogDescription":"","ogImgId":"","twTitle":"","twDescription":"","twImgId":""},"disqusIdentifier":"74045 http://science.kqed.org/quest/?p=74045","disqusUrl":"https://ww2.kqed.org/quest/2015/04/30/diagnosing-diseases-with-origami-microscopes-engineering-is/","disqusTitle":"Diagnosing Diseases With Origami Microscopes","videoEmbed":"http://www.youtube.com/watch?v=k56MVoz9QIc","source":"Engineering","path":"/quest/74045/diagnosing-diseases-with-origami-microscopes-engineering-is","audioTrackLength":null,"parsedContent":[{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003cp>\u003cem>Trouble viewing the video? Stream or download it on \u003ca href=\"http://www.pbslearningmedia.org/resource/727416dd-b83b-4cfb-abbc-27ec2651629b/diagnosing-diseases-with-origami-microscopes/\"> PBS LearningMedia\u003c/a>.\u003c/em>\u003c/p>\n\u003cp>\u003cem>Article by Lauren Farrar\u003c/em>\u003c/p>\n\u003cp>“What if you could drop microscopes literally around the world from an airplane?” Manu Prakash, a professor of bioengineering at Stanford University would often joke with his team.\u003c/p>\n\u003cp>This musing actually heavily influenced the design of their new microscope, a paper origami microscope. They call it a Foldscope. The microscope is printed on waterproof paper. The user punches out the pieces and folds them together to create a fully functional microscope. It works with standard microscope slides and requires no batteries or electricity to operate. You simply hold the Foldscope up to a light source (like the sun) and look through the salt grain-sized lens to view the sample on the slide. The high curvature of the tiny lenses used in the Foldscope allows small objects to be highly magnified. This little invention costs less than a dollar to produce and could have major implications for global health and for science education.\u003c/p>\n\u003cfigure id=\"attachment_74155\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/04/Foldscope_CU_small_white.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2015/04/Foldscope_CU_small_white-300x169.jpg\" alt=\"the Foldscope\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-74155\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">A close up of the Foldscope.\u003c/figcaption>\u003c/figure>\n\u003cp>Prakash grew up in small towns in India where access to healthcare and diagnostic tools, like microscopes, weren’t always easy to come by. He has traveled extensively throughout developing countries and has seen firsthand how diseases like malaria, African sleeping sickness and schistosomiasis can go untreated or are mistreated because patients are not properly diagnosed. Diagnosis of diseases like these usually require a microscope to identify the presence of a particular parasite so that the proper treatment can be administered. But remote or resource poor areas don’t always have access to these heavy, expensive microscopes used in other parts of the world. Standard microscopes are also difficult to transport when doctors travel from village to village to diagnose and treat patients. And even when remote places do have microscopes, there is little support to maintain them or parts available to fix them if they break.\u003c/p>\n\u003cp>\u003c/p>\u003c/div>","attributes":{"named":{},"numeric":[]}},{"type":"component","content":"","name":"ad","attributes":{"named":{"label":"fullwidth"},"numeric":["fullwidth"]}},{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003c/p>\n\u003cp>“Humidity and tropics are terrible for optics… Most of the time when we’re in the field there is fungus growing on the lenses… There were times when we were imaging there, we saw an insect colony come out of the microscope,” Prakash describes some of the challenges of doing field microscopy in remote tropical areas.\u003c/p>\n\u003cp>Prakash was determined to build a cheap, robust, easy-to-use and easy-to-fix microscope that required no power and worked with traditional microscope slides. After prototyping with simple supplies like paper, tape and scissors, he and his team came up with a design that worked. The base level requires no external power source and relies on the sun as the primary light source. The team has since developed multiple versions that allow users to do different kinds of microscopy. For example, there are versions with external light modules, versions of various magnifications, versions that allow for fluorescence microscopy, versions that allow for bright field microscopy, versions that allow for dark field microscopy, and versions that allow the user to project the image on a wall. You can even attach a smartphone to the microscope in order to record images.\u003c/p>\n\u003cp>The Foldscopes are currently undergoing validation studies and clinical trials to be approved as diagnostic tools for diseases including malaria, African sleeping sickness and schistosomiasis.\u003c/p>\n\u003cp>In addition to their potential value for global health, Foldscopes have a strong educational appeal. Prakash wanted to get these microscopes in the hands of children everywhere, so he started the \u003ca href=\"http://www.foldscope.com/\"> Ten Thousand Microscope project\u003c/a>. He wanted to create a network of learners asking questions, so he put a call out for people to submit an idea for an experiment that they would like to do with the Foldscope and then report back on their findings. This idea was so well received that he has shipped approximately 50,000 microscopes to people all around the world. Some of them are already reporting their findings on his \u003ca href=\"http://microcosmos.foldscope.com/\"> website\u003c/a>. He hopes that this project will inspire curiosity and scientific thinking.\u003c/p>\n\u003cp>“Scientific tools need to become common day-to-day objects if we want people to think scientifically,” he says.\u003c/p>\n\u003cp>While Foldscopes used for diagnostic testing come pre-folded to ensure high quality control, the ones sent out for educational purposes through the Ten Thousand Microscope Project are folded and built by the users. He hopes that school children, hackers, and tinkerers all over the world will build on and add to the microscopes. While Prakash isn’t quite dropping these Foldscopes from airplanes, he’s certainly getting them into the hands of children all around the world.\u003c/p>\n\u003cp>\u003c/p>\n\u003cp>This video is part of our \u003cstrong>\u003ca href=\"http://foldscope.woop.ie/\" target=\"_blank\">Engineering Is: Diagnosing Diseases with Origami Microscopes\u003c/a>\u003c/strong> e-book. The e-book explores the science and engineering principles behind Manu Prakash’s Foldscopes project, and includes videos, interactives and media making opportunities. You can find our other e-books at \u003ca href=\"http://blogs.kqed.org/education/e-books/\"> kqed.org/ebooks\u003c/a>.\u003c/p>\n\n\u003c/div>\u003c/p>","attributes":{"named":{},"numeric":[]}}],"link":"/quest/74045/diagnosing-diseases-with-origami-microscopes-engineering-is","authors":["10423"],"categories":["quest_4","quest_8","quest_12"],"tags":["quest_13165","quest_838","quest_12946","quest_13126","quest_13197","quest_13162","quest_12269","quest_3351","quest_13161","quest_1815","quest_1816","quest_13164","quest_13163","quest_2349","quest_2774","quest_3071"],"collections":["quest_13175"],"featImg":"quest_74047","label":"source_quest_74045"},"quest_40277":{"type":"posts","id":"quest_40277","meta":{"index":"posts_1591205157","site":"quest","id":"40277","score":null,"sort":[1347388245000]},"guestAuthors":[],"slug":"x-ray-microscope-seeing-cells-in-3-d","title":"X-ray Microscope: Seeing Cells in 3-D","publishDate":1347388245,"format":"video","headTitle":"QUEST | KQED Science","labelTerm":{},"content":"\u003cfigure id=\"attachment_40297\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_Scope_LeGros1.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_Scope_LeGros1.jpg\" alt=\"\" title=\"XrayMicroscope_Day2_Scope_LeGros\" width=\"300\" height=\"169\" class=\"size-full wp-image-40297\" srcset=\"https://ww2.kqed.org/app/uploads/sites/39/2012/06/XrayMicroscope_Day2_Scope_LeGros1.jpg 640w, https://ww2.kqed.org/app/uploads/sites/39/2012/06/XrayMicroscope_Day2_Scope_LeGros1-400x225.jpg 400w\" sizes=\"(max-width: 300px) 100vw, 300px\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">Physicist Mark LeGros spent three years building this microscope which uses x-rays to take 3-D images of biological cells. \u003c/figcaption>\u003c/figure>\n\u003cp>Mark LeGros, who spent three years building the world's first x-ray microscope dedicated to cell biology, greeted me at the \u003ca href=\"http://www.lbl.gov/\">Lawrence Berkeley National Laboratory \u003c/a>(LBNL) with a hearty handshake, effusive smile and an accent reminiscent of his New Zealand roots. \u003c/p>\n\u003cp>I spent an hour in April talking with him about the challenges and rewards of creating a powerful, state-of-the art microscope which can capture a truer, more accurate picture of a whole cell and its nucleus, mitochondria and other tiny, essential cellular structures. \u003c/p>\n\u003cp>I asked him to take me back to that moment in 2009 when he and the diverse team of researchers, including biologists, physicists, chemists and computer scientists, who comprise the \u003ca href=\"http://ncxt.lbl.gov\">National Center of X-ray Tomography\u003c/a> at LBNL, powered up the microscope and waited anxiously for the first x-ray images of a yeast cell, the biological specimen they chose to test the capabilities of their new imaging tool. \u003c/p>\n\u003cfigure id=\"attachment_40309\" class=\"wp-caption alignright\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_MarkLeGros1.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_MarkLeGros1-300x169.jpg\" alt=\"\" title=\"XrayMicroscope_Day2_MarkLeGros\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40309\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">Physicist Mark LeGros smiles for the camera while Bertrand Cinquin, a postdoctoral researcher, waits outside the lab containing the x-ray microscope. Photo by Sheraz Sadiq / QUEST.\u003c/figcaption>\u003c/figure>\n\u003cp>\"I wasn’t sure exactly just how well this was gonna work out. But it turned out that it was beautiful...and everybody associated with the project was stunned with the degree of detail that it revealed about the internal structure of a cell and the chromatin, which is the genetic material of the cell,\" he said. \u003c/p>\n\u003cp>It's important to note that this powerful device does not render obsolete other imaging techniques, including \u003ca href=\"http://en.wikipedia.org/wiki/Optical_microscope\">light microscopy\u003c/a> and \u003ca href=\"http://en.wikipedia.org/wiki/Electron_microscope\">electron microscopy\u003c/a>. For one thing, the cell sample is frozen to hold the structures in place and protect them from the x-rays which have much greater penetrating power than visible light because x-rays have shorter wavelengths than light rays. So it can't capture dynamic activity of cells moving in real-time, unlike an optical or light microscope. Also, while the resolution possible with the x-ray microscope is five times greater than a light microscope, an electron microscope, which uses electrons to penetrate ultra-thin sections of cells, provides better resolution than the x-ray microscope. \u003c/p>\n\u003cfigure id=\"attachment_40298\" class=\"wp-caption alignright\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_Larabell_Scope1.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_Larabell_Scope1-300x169.jpg\" alt=\"\" title=\"XrayMicroscope_Day2_Larabell_Scope\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40298\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">Carolyn Larabell looks at images taken with the x-ray microscope while Bertrand Cinquin loads a sample of cells into the microscope. Photo by Sheraz Sadiq / QUEST.\u003c/figcaption>\u003c/figure>\n\u003cp>Nonetheless, unlike an electron microscope, the x-ray microscope allows scientists to image a whole cell in its native state, with the only preparation being the freezing of the cell sample. Also, many of the classic textbook images of cells that were taken with electron microscopes which required the cells to be dehydrated and stained with heavy metals to bind the electrons to the cell and generate a rather grainy image. Since a cell is 70% water, dehydration and staining can degrade delicate cellular structures and thus make it hard to accurately visualize them. \u003c/p>\n\u003cp>[ad fullwidth]\u003c/p>\n\u003cp>In addition, the x-ray microscope uses the technique of tomography to faithfully reconstruct the volume of a cell in three dimensions, instead of a flat, 2-D image of it. \u003c/p>\n\u003cp>Here, a sample of cells is placed in the microscope and rotated 360 degrees. While it rotates, x-rays illuminate the carbon and nitrogen present in all biological cells and black and white images then display the absorption of the x-rays by the organic material in every part of the cell. All of this happens in about 30 seconds. Computer software then allows the team to process, in a mere five to ten minutes, the individual x-ray images and reconstruct a 3-D portrait of the cell which is possible since the cell has been imaged from every conceivable angle, allowing not only the shapes of its structures to be seen but also the amount of biological material such as the DNA packed into the cell's nucleus.\u003c/p>\n\u003cfigure id=\"attachment_40394\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/07/CLarabell_olfactory-neuron.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/07/CLarabell_olfactory-neuron-300x169.jpg\" alt=\"\" title=\"CLarabell_olfactory neuron\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40394\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">A cross-section of stacked x-ray images taken of a mouse olfactory neuron, with the dark regions indicating the inactive genetic material and the light regions indicating the active genetic material within the nucleus. Image courtesy of Carolyn Larabell, PhD.\u003c/figcaption>\u003c/figure>\n\u003cp>This novel form of 3-D imaging is revealing new insight into the organization of the active genes - the euchromatin - and the silenced genes - the heterochromatin - in the nucleus. \u003c/p>\n\u003cp>\"So we can see now for the first time that the heterochromatin, or those silenced genes, are more crowded than the euchromatin regions. And we can actually quantify that and say that it’s 28% more crowded,\" said \u003ca href=\"http://www.lbl.gov/wonder/larabell.html\">Carolyn Larabell\u003c/a>, a microbiologist and the Director of the National Center for X-ray Tomography. \u003c/p>\n\u003cfigure id=\"attachment_40303\" class=\"wp-caption alignright\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/605B_CLarabell_mouse_olfactory_neuron.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/605B_CLarabell_mouse_olfactory_neuron-300x169.jpg\" alt=\"\" title=\"605B_CLarabell_mouse_olfactory_neuron\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40303\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">The stack of x-ray images taken of the mouse olfactory neuron is then processed by computer software and turned into this striking 3-D illustration of the cell, with the nucleus shown in blue. Image courtesy of Carolyn Larabell, PhD.\u003c/figcaption>\u003c/figure>\n\u003cp>So why is it important to quantify the amount of active genes versus silenced genes in a cell's nucleus? For one thing, in certain diseases like cancer, previously silenced genes become active and the nucleus grows larger. So perhaps this new level of insight can shed light on the progression of cancer and perhaps even offer an early tool for its onset, well before symptoms appear. A three-dimensional window into cells may also lead to the development of better designed and consequently, more effective drug treatments. \u003c/p>\n\u003cfigure id=\"attachment_40325\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day1_Larabell1.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day1_Larabell1-300x169.jpg\" alt=\"\" title=\"XrayMicroscope_Day1_Larabell\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40325\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">Carolyn Larabell is a microbiologist and the Director of the National Center of X-ray Tomography at LBNL. Photo by Sheraz Sadiq / QUEST. \u003c/figcaption>\u003c/figure>\n\u003cp>\"It’s important to see these structures in 3-D (because) you want to test drugs on cells to see if they’re having the effect that you think they are,\" said Larabell. \"If you get a single section, you have just a very thin window on what might be happening to that cell. (So) you want to see what’s happening throughout the entire cell,\" she added. \u003c/p>\n\u003cp>Whether it's the life cycle of the deadly malaria parasite or the breakdown of sugars from plants in the production of biofuels, this exciting new imaging tool is providing a richer understanding of important biological processes. \u003c/p>\n\u003cp>[ad floatright]\u003c/p>\n\u003cp>But as ingenious as this microscope is, it can't compare to the ingenuity and complexity of the microscopic world of cells which we've strained to bring into lucid focus for hundreds of years, ever since Robert Hooke's 17th-century descriptions of the tiny structures in a section of cork, which he likened to the modest chambers, or cells, of pious monks. \u003c/p>\n\n","blocks":[],"excerpt":"At the Lawrence Berkeley National Laboratory, scientists are using a cutting-edge microscope, the first of its kind in the world, to image whole cells in 3-D with the penetrating power of x-rays. The new images generated by the microscope are offering a deeper, more precise understanding of cellular structures and how they change with diseases. ","status":"publish","parent":0,"modified":1457566458,"stats":{"hasAudio":false,"hasVideo":false,"hasChartOrMap":false,"iframeSrcs":[],"hasGoogleForm":false,"hasGallery":false,"hasHearkenModule":false,"hasPolis":false,"paragraphCount":16,"wordCount":1115},"headData":{"title":"X-ray Microscope: Seeing Cells in 3-D | KQED","description":"At the Lawrence Berkeley National Laboratory, scientists are using a cutting-edge microscope, the first of its kind in the world, to image whole cells in 3-D with the penetrating power of x-rays. The new images generated by the microscope are offering a deeper, more precise understanding of cellular structures and how they change with diseases. ","ogTitle":"","ogDescription":"","ogImgId":"","twTitle":"","twDescription":"","twImgId":""},"disqusIdentifier":"40277 http://science.kqed.org/quest/?post_type=videos&p=40277","disqusUrl":"https://ww2.kqed.org/quest/2012/09/11/x-ray-microscope-seeing-cells-in-3-d/","disqusTitle":"X-ray Microscope: Seeing Cells in 3-D","videoEmbed":"https://www.youtube.com/watch?v=WcO7l2DdgEA","source":"Biology","sourceUrl":"https://ww2.kqed.org/quest/category/biology/","path":"/quest/40277/x-ray-microscope-seeing-cells-in-3-d","audioTrackLength":null,"parsedContent":[{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003cfigure id=\"attachment_40297\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_Scope_LeGros1.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_Scope_LeGros1.jpg\" alt=\"\" title=\"XrayMicroscope_Day2_Scope_LeGros\" width=\"300\" height=\"169\" class=\"size-full wp-image-40297\" srcset=\"https://ww2.kqed.org/app/uploads/sites/39/2012/06/XrayMicroscope_Day2_Scope_LeGros1.jpg 640w, https://ww2.kqed.org/app/uploads/sites/39/2012/06/XrayMicroscope_Day2_Scope_LeGros1-400x225.jpg 400w\" sizes=\"(max-width: 300px) 100vw, 300px\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">Physicist Mark LeGros spent three years building this microscope which uses x-rays to take 3-D images of biological cells. \u003c/figcaption>\u003c/figure>\n\u003cp>Mark LeGros, who spent three years building the world's first x-ray microscope dedicated to cell biology, greeted me at the \u003ca href=\"http://www.lbl.gov/\">Lawrence Berkeley National Laboratory \u003c/a>(LBNL) with a hearty handshake, effusive smile and an accent reminiscent of his New Zealand roots. \u003c/p>\n\u003cp>I spent an hour in April talking with him about the challenges and rewards of creating a powerful, state-of-the art microscope which can capture a truer, more accurate picture of a whole cell and its nucleus, mitochondria and other tiny, essential cellular structures. \u003c/p>\n\u003cp>I asked him to take me back to that moment in 2009 when he and the diverse team of researchers, including biologists, physicists, chemists and computer scientists, who comprise the \u003ca href=\"http://ncxt.lbl.gov\">National Center of X-ray Tomography\u003c/a> at LBNL, powered up the microscope and waited anxiously for the first x-ray images of a yeast cell, the biological specimen they chose to test the capabilities of their new imaging tool. \u003c/p>\n\u003cfigure id=\"attachment_40309\" class=\"wp-caption alignright\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_MarkLeGros1.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_MarkLeGros1-300x169.jpg\" alt=\"\" title=\"XrayMicroscope_Day2_MarkLeGros\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40309\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">Physicist Mark LeGros smiles for the camera while Bertrand Cinquin, a postdoctoral researcher, waits outside the lab containing the x-ray microscope. Photo by Sheraz Sadiq / QUEST.\u003c/figcaption>\u003c/figure>\n\u003cp>\"I wasn’t sure exactly just how well this was gonna work out. But it turned out that it was beautiful...and everybody associated with the project was stunned with the degree of detail that it revealed about the internal structure of a cell and the chromatin, which is the genetic material of the cell,\" he said. \u003c/p>\n\u003cp>It's important to note that this powerful device does not render obsolete other imaging techniques, including \u003ca href=\"http://en.wikipedia.org/wiki/Optical_microscope\">light microscopy\u003c/a> and \u003ca href=\"http://en.wikipedia.org/wiki/Electron_microscope\">electron microscopy\u003c/a>. For one thing, the cell sample is frozen to hold the structures in place and protect them from the x-rays which have much greater penetrating power than visible light because x-rays have shorter wavelengths than light rays. So it can't capture dynamic activity of cells moving in real-time, unlike an optical or light microscope. Also, while the resolution possible with the x-ray microscope is five times greater than a light microscope, an electron microscope, which uses electrons to penetrate ultra-thin sections of cells, provides better resolution than the x-ray microscope. \u003c/p>\n\u003cfigure id=\"attachment_40298\" class=\"wp-caption alignright\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_Larabell_Scope1.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day2_Larabell_Scope1-300x169.jpg\" alt=\"\" title=\"XrayMicroscope_Day2_Larabell_Scope\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40298\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">Carolyn Larabell looks at images taken with the x-ray microscope while Bertrand Cinquin loads a sample of cells into the microscope. Photo by Sheraz Sadiq / QUEST.\u003c/figcaption>\u003c/figure>\n\u003cp>Nonetheless, unlike an electron microscope, the x-ray microscope allows scientists to image a whole cell in its native state, with the only preparation being the freezing of the cell sample. Also, many of the classic textbook images of cells that were taken with electron microscopes which required the cells to be dehydrated and stained with heavy metals to bind the electrons to the cell and generate a rather grainy image. Since a cell is 70% water, dehydration and staining can degrade delicate cellular structures and thus make it hard to accurately visualize them. \u003c/p>\n\u003cp>\u003c/p>\u003c/div>","attributes":{"named":{},"numeric":[]}},{"type":"component","content":"","name":"ad","attributes":{"named":{"label":"fullwidth"},"numeric":["fullwidth"]}},{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003c/p>\n\u003cp>In addition, the x-ray microscope uses the technique of tomography to faithfully reconstruct the volume of a cell in three dimensions, instead of a flat, 2-D image of it. \u003c/p>\n\u003cp>Here, a sample of cells is placed in the microscope and rotated 360 degrees. While it rotates, x-rays illuminate the carbon and nitrogen present in all biological cells and black and white images then display the absorption of the x-rays by the organic material in every part of the cell. All of this happens in about 30 seconds. Computer software then allows the team to process, in a mere five to ten minutes, the individual x-ray images and reconstruct a 3-D portrait of the cell which is possible since the cell has been imaged from every conceivable angle, allowing not only the shapes of its structures to be seen but also the amount of biological material such as the DNA packed into the cell's nucleus.\u003c/p>\n\u003cfigure id=\"attachment_40394\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/07/CLarabell_olfactory-neuron.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/07/CLarabell_olfactory-neuron-300x169.jpg\" alt=\"\" title=\"CLarabell_olfactory neuron\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40394\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">A cross-section of stacked x-ray images taken of a mouse olfactory neuron, with the dark regions indicating the inactive genetic material and the light regions indicating the active genetic material within the nucleus. Image courtesy of Carolyn Larabell, PhD.\u003c/figcaption>\u003c/figure>\n\u003cp>This novel form of 3-D imaging is revealing new insight into the organization of the active genes - the euchromatin - and the silenced genes - the heterochromatin - in the nucleus. \u003c/p>\n\u003cp>\"So we can see now for the first time that the heterochromatin, or those silenced genes, are more crowded than the euchromatin regions. And we can actually quantify that and say that it’s 28% more crowded,\" said \u003ca href=\"http://www.lbl.gov/wonder/larabell.html\">Carolyn Larabell\u003c/a>, a microbiologist and the Director of the National Center for X-ray Tomography. \u003c/p>\n\u003cfigure id=\"attachment_40303\" class=\"wp-caption alignright\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/605B_CLarabell_mouse_olfactory_neuron.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/605B_CLarabell_mouse_olfactory_neuron-300x169.jpg\" alt=\"\" title=\"605B_CLarabell_mouse_olfactory_neuron\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40303\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">The stack of x-ray images taken of the mouse olfactory neuron is then processed by computer software and turned into this striking 3-D illustration of the cell, with the nucleus shown in blue. Image courtesy of Carolyn Larabell, PhD.\u003c/figcaption>\u003c/figure>\n\u003cp>So why is it important to quantify the amount of active genes versus silenced genes in a cell's nucleus? For one thing, in certain diseases like cancer, previously silenced genes become active and the nucleus grows larger. So perhaps this new level of insight can shed light on the progression of cancer and perhaps even offer an early tool for its onset, well before symptoms appear. A three-dimensional window into cells may also lead to the development of better designed and consequently, more effective drug treatments. \u003c/p>\n\u003cfigure id=\"attachment_40325\" class=\"wp-caption alignleft\" style=\"max-width: 300px\">\u003ca href=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day1_Larabell1.jpg\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2012/06/XrayMicroscope_Day1_Larabell1-300x169.jpg\" alt=\"\" title=\"XrayMicroscope_Day1_Larabell\" width=\"300\" height=\"169\" class=\"size-thumbnail wp-image-40325\">\u003c/a>\u003cfigcaption class=\"wp-caption-text\">Carolyn Larabell is a microbiologist and the Director of the National Center of X-ray Tomography at LBNL. Photo by Sheraz Sadiq / QUEST. \u003c/figcaption>\u003c/figure>\n\u003cp>\"It’s important to see these structures in 3-D (because) you want to test drugs on cells to see if they’re having the effect that you think they are,\" said Larabell. \"If you get a single section, you have just a very thin window on what might be happening to that cell. (So) you want to see what’s happening throughout the entire cell,\" she added. \u003c/p>\n\u003cp>Whether it's the life cycle of the deadly malaria parasite or the breakdown of sugars from plants in the production of biofuels, this exciting new imaging tool is providing a richer understanding of important biological processes. \u003c/p>\n\u003cp>\u003c/p>\u003c/div>","attributes":{"named":{},"numeric":[]}},{"type":"component","content":"","name":"ad","attributes":{"named":{"label":"floatright"},"numeric":["floatright"]}},{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003c/p>\n\u003cp>But as ingenious as this microscope is, it can't compare to the ingenuity and complexity of the microscopic world of cells which we've strained to bring into lucid focus for hundreds of years, ever since Robert Hooke's 17th-century descriptions of the tiny structures in a section of cork, which he likened to the modest chambers, or cells, of pious monks. \u003c/p>\n\n\u003c/div>\u003c/p>","attributes":{"named":{},"numeric":[]}}],"link":"/quest/40277/x-ray-microscope-seeing-cells-in-3-d","authors":["6176"],"categories":["quest_4","quest_12","quest_3422","quest_3233"],"tags":["quest_475","quest_1626","quest_11250","quest_1815","quest_13","quest_11251","quest_2893","quest_11248","quest_3071"],"featImg":"quest_40303","label":"source_quest_40277"},"quest_4165":{"type":"posts","id":"quest_4165","meta":{"index":"posts_1591205157","site":"quest","id":"4165","score":null,"sort":[1257186212000]},"guestAuthors":[],"slug":"50-years-later-still-plenty-of-room-at-the-bottom","title":"50 Years Later, Still Plenty of Room at the Bottom","publishDate":1257186212,"format":"standard","headTitle":"QUEST | KQED Science","labelTerm":{"site":"quest"},"content":"\u003cp>\u003cspan class=\"left\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2009/11/graphene.jpg\">\u003cem>Lawrence Berkeley Lab's TEAM 0.5 is capable of resolving individual carbon atoms in the honeycomb crystal structure of graphene. See QUEST's video \u003ca href=\"http://ww2.kqed.org/quest/video/the-worlds-most-powerful-microscope\">The World's Most Powerful Microscope\u003c/a> for more information. Image source: Nano Letters\u003c/em>\u003c/span>The twentieth century’s most important physicist after Albert Einstein is almost certainly \u003ca href=\"http://en.wikipedia.org/wiki/Feynman\">Richard Feynman\u003c/a>. Known as much for his eccentricities as for his brilliance, he spent his adolescent spare time picking locks, translated Mayan hieroglyphics as an adult, and was one of the few people brash enough to attempt viewing the U.S.’s first atomic bomb test without protective sunglasses. Feynman’s chief scientific contribution was the development of \u003ca href=\"http://en.wikipedia.org/wiki/Quantum_electrodynamics\">QED\u003c/a>, a fundamental and astonishingly accurate description of electricity and magnetism. However, he was also a champion of the practical, and in 1959 gave a gave a prophetic speech at Caltech to his colleagues entitled, “\u003ca href=\"http://www.me.ucsb.edu/course_pages/course_pages_f09/me141a/plenty_of_room.pdf\">There’s Plenty of Room at the Bottom\u003c/a>.” The speech described a rich world of possibilities that could arise if we only applied ourselves toward controlling matter on smaller and smaller scales.\u003c/p>\n\u003cp>Fifty years later, a new field of \u003ca href=\"http://ww2.kqed.org/quest/video/nanotechnology-takes-off\">nanotechnology\u003c/a> has exploded. At the cutting edge, researchers are successfully manufacturing everything from \u003ca href=\"http://www.almaden.ibm.com/vis/stm/images/stm10.jpg\">corporate logos\u003c/a> to \u003ca href=\"http://www.physics.berkeley.edu/research/zettl/projects/nanoradio/radio.html\">radios\u003c/a> that are all small enough to be stacked end-to-end perhaps a million items long across the proverbial head of a pin. The advent of personal computers and smart phones has brought the power of such miniaturization into sharp focus for the general public. In a very real sense, we have all become bottom feeders. Below is a brief progress report on the state of the field.\u003c/p>\n\u003cp>\u003cstrong>Microscopes: \u003c/strong>The old adage “seeing is believing” was not lost on Feynman back in the late fifties. He noted that many of the most fundamental questions in biology could be readily solved if we only had the ability to see the molecules directly. Today, new inventions such as the \u003ca href=\"http://en.wikipedia.org/wiki/Scanning_tunneling_microscope\">scanning tunneling microscope\u003c/a> (STM), the \u003ca href=\"http://en.wikipedia.org/wiki/Atomic_force_microscope\">atomic force microscope\u003c/a> (AFM), and the \u003ca href=\"http://en.wikipedia.org/wiki/Transmission_electron_microscopy\">transmission electron microscope\u003c/a> (TEM) have all achieved resolution at the scale where individual atoms can actually be seen and manipulated.\u003c/p>\n\u003cp>\u003cstrong>Miniature Motors: \u003c/strong>Perhaps the speech’s most imaginative scenario, due to Feynman’s friend (and graduate student) Albert Hibbs, was the concept of being able to “swallow the surgeon.” Feynman imagined that we might some day be able to construct robots capable of repairing or investigating the inner reaches of an ailing patient’s body. Mixing engineering and biology like this can run quickly into thorny ethical questions. Nevertheless, interesting progress has been made. Researchers in Alex Zettl’s group at UC Berkeley have recently constructed a nano motor, for example.\u003c/p>\n\u003cp>\u003cstrong>Information Storage: \u003c/strong>Using order-of-magnitude arguments, Feynman argued that the Encyclopedia Britannica could be squeezed into a pin’s area if the text were reduced by a factor of 25,000. He offered a $1,000 prize to the first person capable of printing one page of any book at this scale. Tom Newman, a graduate student at Stanford, \u003ca href=\"http://calteches.library.caltech.edu/597/2/Tale.pdf\">first accomplished this\u003c/a> in 1986 with an impressive reprinting of the first page of Dickens’ classic \u003cem>\u003ca href=\"http://www.amazon.com/Tale-Two-Cities-Charles-Dickens/dp/1448625025/ref=sr_1_1?ie=UTF8&s=books&qid=1257154831&sr=1-1\">A Tale of Two Cities\u003c/a>\u003c/em>. Today, you can buy the book in its entirety for only 1.9 megabytes. For a high-end smart phone with 30 gigabytes of memory, you could perhaps hold 15,000 books within the palm of your hand. Not bad.\u003c/p>\n\u003cp>[ad fullwidth]\u003c/p>\n\u003cp>Then again, at the extreme limit, Feynman also reasoned that you ought to be able to squeeze the text of every book that has ever been written (now more than 32 million titles according the \u003ca href=\"http://www.loc.gov/about/facts.html\">Library of Congress\u003c/a>) within the confines of a single speck of dust. We still have a long way to go.\u003c/p>\n\u003cp>\u003c/p>\n\u003cp> 37.8768 -122.251\u003c/p>\n\n","blocks":[],"excerpt":"50 years ago, eminent physicist Richard Feynman gave a gave a prophetic speech at Caltech entitled, \"There's Plenty of Room at the Bottom.\" The speech described a rich world of possibilities that could arise if we only applied ourselves toward controlling matter on smaller and smaller scales.","status":"publish","parent":0,"modified":1443833609,"stats":{"hasAudio":false,"hasVideo":false,"hasChartOrMap":false,"iframeSrcs":[],"hasGoogleForm":false,"hasGallery":false,"hasHearkenModule":false,"hasPolis":false,"paragraphCount":9,"wordCount":635},"headData":{"title":"50 Years Later, Still Plenty of Room at the Bottom | KQED","description":"50 years ago, eminent physicist Richard Feynman gave a gave a prophetic speech at Caltech entitled, "There's Plenty of Room at the Bottom." The speech described a rich world of possibilities that could arise if we only applied ourselves toward controlling matter on smaller and smaller scales.","ogTitle":"","ogDescription":"","ogImgId":"","twTitle":"","twDescription":"","twImgId":""},"disqusIdentifier":"4165 http://www.kqed.org/quest/blog/?p=4165","disqusUrl":"https://ww2.kqed.org/quest/2009/11/02/50-years-later-still-plenty-of-room-at-the-bottom/","disqusTitle":"50 Years Later, Still Plenty of Room at the Bottom","path":"/quest/4165/50-years-later-still-plenty-of-room-at-the-bottom","audioTrackLength":null,"parsedContent":[{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003cp>\u003cspan class=\"left\">\u003cimg src=\"http://ww2.kqed.org/quest/wp-content/uploads/sites/39/2009/11/graphene.jpg\">\u003cem>Lawrence Berkeley Lab's TEAM 0.5 is capable of resolving individual carbon atoms in the honeycomb crystal structure of graphene. See QUEST's video \u003ca href=\"http://ww2.kqed.org/quest/video/the-worlds-most-powerful-microscope\">The World's Most Powerful Microscope\u003c/a> for more information. Image source: Nano Letters\u003c/em>\u003c/span>The twentieth century’s most important physicist after Albert Einstein is almost certainly \u003ca href=\"http://en.wikipedia.org/wiki/Feynman\">Richard Feynman\u003c/a>. Known as much for his eccentricities as for his brilliance, he spent his adolescent spare time picking locks, translated Mayan hieroglyphics as an adult, and was one of the few people brash enough to attempt viewing the U.S.’s first atomic bomb test without protective sunglasses. Feynman’s chief scientific contribution was the development of \u003ca href=\"http://en.wikipedia.org/wiki/Quantum_electrodynamics\">QED\u003c/a>, a fundamental and astonishingly accurate description of electricity and magnetism. However, he was also a champion of the practical, and in 1959 gave a gave a prophetic speech at Caltech to his colleagues entitled, “\u003ca href=\"http://www.me.ucsb.edu/course_pages/course_pages_f09/me141a/plenty_of_room.pdf\">There’s Plenty of Room at the Bottom\u003c/a>.” The speech described a rich world of possibilities that could arise if we only applied ourselves toward controlling matter on smaller and smaller scales.\u003c/p>\n\u003cp>Fifty years later, a new field of \u003ca href=\"http://ww2.kqed.org/quest/video/nanotechnology-takes-off\">nanotechnology\u003c/a> has exploded. At the cutting edge, researchers are successfully manufacturing everything from \u003ca href=\"http://www.almaden.ibm.com/vis/stm/images/stm10.jpg\">corporate logos\u003c/a> to \u003ca href=\"http://www.physics.berkeley.edu/research/zettl/projects/nanoradio/radio.html\">radios\u003c/a> that are all small enough to be stacked end-to-end perhaps a million items long across the proverbial head of a pin. The advent of personal computers and smart phones has brought the power of such miniaturization into sharp focus for the general public. In a very real sense, we have all become bottom feeders. Below is a brief progress report on the state of the field.\u003c/p>\n\u003cp>\u003cstrong>Microscopes: \u003c/strong>The old adage “seeing is believing” was not lost on Feynman back in the late fifties. He noted that many of the most fundamental questions in biology could be readily solved if we only had the ability to see the molecules directly. Today, new inventions such as the \u003ca href=\"http://en.wikipedia.org/wiki/Scanning_tunneling_microscope\">scanning tunneling microscope\u003c/a> (STM), the \u003ca href=\"http://en.wikipedia.org/wiki/Atomic_force_microscope\">atomic force microscope\u003c/a> (AFM), and the \u003ca href=\"http://en.wikipedia.org/wiki/Transmission_electron_microscopy\">transmission electron microscope\u003c/a> (TEM) have all achieved resolution at the scale where individual atoms can actually be seen and manipulated.\u003c/p>\n\u003cp>\u003cstrong>Miniature Motors: \u003c/strong>Perhaps the speech’s most imaginative scenario, due to Feynman’s friend (and graduate student) Albert Hibbs, was the concept of being able to “swallow the surgeon.” Feynman imagined that we might some day be able to construct robots capable of repairing or investigating the inner reaches of an ailing patient’s body. Mixing engineering and biology like this can run quickly into thorny ethical questions. Nevertheless, interesting progress has been made. Researchers in Alex Zettl’s group at UC Berkeley have recently constructed a nano motor, for example.\u003c/p>\n\u003cp>\u003cstrong>Information Storage: \u003c/strong>Using order-of-magnitude arguments, Feynman argued that the Encyclopedia Britannica could be squeezed into a pin’s area if the text were reduced by a factor of 25,000. He offered a $1,000 prize to the first person capable of printing one page of any book at this scale. Tom Newman, a graduate student at Stanford, \u003ca href=\"http://calteches.library.caltech.edu/597/2/Tale.pdf\">first accomplished this\u003c/a> in 1986 with an impressive reprinting of the first page of Dickens’ classic \u003cem>\u003ca href=\"http://www.amazon.com/Tale-Two-Cities-Charles-Dickens/dp/1448625025/ref=sr_1_1?ie=UTF8&s=books&qid=1257154831&sr=1-1\">A Tale of Two Cities\u003c/a>\u003c/em>. Today, you can buy the book in its entirety for only 1.9 megabytes. For a high-end smart phone with 30 gigabytes of memory, you could perhaps hold 15,000 books within the palm of your hand. Not bad.\u003c/p>\n\u003cp>\u003c/p>\u003c/div>","attributes":{"named":{},"numeric":[]}},{"type":"component","content":"","name":"ad","attributes":{"named":{"label":"fullwidth"},"numeric":["fullwidth"]}},{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003c/p>\n\u003cp>Then again, at the extreme limit, Feynman also reasoned that you ought to be able to squeeze the text of every book that has ever been written (now more than 32 million titles according the \u003ca href=\"http://www.loc.gov/about/facts.html\">Library of Congress\u003c/a>) within the confines of a single speck of dust. We still have a long way to go.\u003c/p>\n\u003cp>\u003c/p>\n\u003cp> 37.8768 -122.251\u003c/p>\n\n\u003c/div>\u003c/p>","attributes":{"named":{},"numeric":[]}}],"link":"/quest/4165/50-years-later-still-plenty-of-room-at-the-bottom","authors":["10205"],"categories":["quest_8","quest_16"],"tags":["quest_76","quest_465","quest_1630","quest_1815","quest_1913","quest_13205","quest_2343","quest_2430","quest_2800","quest_2867","quest_2899"],"label":"quest"},"quest_17469":{"type":"posts","id":"quest_17469","meta":{"index":"posts_1591205157","site":"quest","id":"17469","score":null,"sort":[1238554200000]},"guestAuthors":[],"slug":"the-worlds-most-powerful-microscope","title":"The World's Most Powerful Microscope","publishDate":1238554200,"format":"video","headTitle":"QUEST | KQED Science","labelTerm":{"site":"quest"},"content":"\u003cp>Lawrence Berkeley National Lab recently turned on a $27 million electron microscope. Its ability to make images to a resolution half the width of a hydrogen atom made it the most powerful microscope in the world.\u003c/p>\n\u003cp>\u003cem>Learn more about this \u003ca href=\"http://ww2.kqed.org/quest/2009/03/30/producers-notes-worlds-most-powerful-microscope/\" target=\"_blank\">microscope and the video\u003c/a> with the Producer's Notes.\u003cbr>\n\u003c/em>\u003c/p>\n\u003cp>[ad fullwidth]\u003c/p>\u003cp>\u003c/p>\n","blocks":[],"excerpt":"Lawrence Berkeley National Lab recently turned on a $27 million electron microscope. Its ability to make images to a resolution half the width of a hydrogen atom made it the most powerful microscope in the world.","status":"publish","parent":0,"modified":1457790839,"stats":{"hasAudio":false,"hasVideo":false,"hasChartOrMap":false,"iframeSrcs":[],"hasGoogleForm":false,"hasGallery":false,"hasHearkenModule":false,"hasPolis":false,"paragraphCount":4,"wordCount":51},"headData":{"title":"The World's Most Powerful Microscope | KQED","description":"Lawrence Berkeley National Lab recently turned on a $27 million electron microscope. Its ability to make images to a resolution half the width of a hydrogen atom made it the most powerful microscope in the world.","ogTitle":"","ogDescription":"","ogImgId":"","twTitle":"","twDescription":"","twImgId":""},"disqusIdentifier":"17469 http://science.kqed.org/quest/video/the-worlds-most-powerful-microscope/","disqusUrl":"https://ww2.kqed.org/quest/2009/03/31/the-worlds-most-powerful-microscope/","disqusTitle":"The World's Most Powerful Microscope","videoEmbed":"https://www.youtube.com/watch?v=sCYX_XQgnSA","path":"/quest/17469/the-worlds-most-powerful-microscope","audioTrackLength":null,"parsedContent":[{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003cp>Lawrence Berkeley National Lab recently turned on a $27 million electron microscope. Its ability to make images to a resolution half the width of a hydrogen atom made it the most powerful microscope in the world.\u003c/p>\n\u003cp>\u003cem>Learn more about this \u003ca href=\"http://ww2.kqed.org/quest/2009/03/30/producers-notes-worlds-most-powerful-microscope/\" target=\"_blank\">microscope and the video\u003c/a> with the Producer's Notes.\u003cbr>\n\u003c/em>\u003c/p>\n\u003cp>\u003c/p>\u003c/div>","attributes":{"named":{},"numeric":[]}},{"type":"component","content":"","name":"ad","attributes":{"named":{"label":"fullwidth"},"numeric":["fullwidth"]}},{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003c/p>\u003cp>\u003c/p>\n\u003c/div>\u003c/p>","attributes":{"named":{},"numeric":[]}}],"link":"/quest/17469/the-worlds-most-powerful-microscope","authors":["6186","10206"],"categories":["quest_8","quest_16","quest_3422","quest_3233"],"tags":["quest_11131","quest_3351","quest_1626","quest_1815","quest_1816","quest_2141","quest_2349","quest_13","quest_2893"],"label":"quest"},"quest_1895":{"type":"posts","id":"quest_1895","meta":{"index":"posts_1591205157","site":"quest","id":"1895","score":null,"sort":[1238441687000]},"guestAuthors":[],"slug":"producers-notes-worlds-most-powerful-microscope","title":"Producer's Notes: World's Most Powerful Microscope","publishDate":1238441687,"format":"video","headTitle":"QUEST | KQED Science","labelTerm":{"site":"quest"},"content":"\u003cp>Today QUEST takes you behind the scenes to see the most powerful microscope in the world, which happens to be in our very own backyard in Berkeley. This transmission electron microscope lives at the \u003ca href=\"http://ncem.lbl.gov/\">National Center for Electron Microscopy\u003c/a>, at the Lawrence Berkeley National Lab. The microscope can produce images of things that are the size of half an atom of hydrogen. And hydrogen has the smallest atoms of any element – so that's pretty small. \u003c/p>\n\u003cp>The microscope is so big that it was hauled into the Center on a crane. It's housed in its own room, which is insulated to maintain an ideal temperature, and it's mounted on springs to isolate it from vibrations that make images blurry. \u003c/p>\n\u003cp>The \u003ca href=\"http://ncem.lbl.gov/TEAM-project/index.html\">TEAM 0.5\u003c/a>, as the microscope is called, excels at producing clear images of atoms sitting side by side. This makes it very useful for the scientists who investigate the properties of the materials that we use to build everyday objects like computers and airplanes. In fact, the images they produce with the microscope may one day help build stronger, lighter airplanes, and smaller, faster computers. \u003c/p>\n\u003cp>[ad fullwidth]\u003c/p>\u003cp>\u003c/p>\n","blocks":[],"excerpt":"Today QUEST takes you behind the scenes to see the most powerful microscope in the world, which happens to be in our very own backyard in Berkeley.","status":"publish","parent":0,"modified":1457790762,"stats":{"hasAudio":false,"hasVideo":false,"hasChartOrMap":false,"iframeSrcs":[],"hasGoogleForm":false,"hasGallery":false,"hasHearkenModule":false,"hasPolis":false,"paragraphCount":5,"wordCount":190},"headData":{"title":"Producer's Notes: World's Most Powerful Microscope | KQED","description":"Today QUEST takes you behind the scenes to see the most powerful microscope in the world, which happens to be in our very own backyard in Berkeley.","ogTitle":"","ogDescription":"","ogImgId":"","twTitle":"","twDescription":"","twImgId":""},"disqusIdentifier":"1895 http://www.kqed.org/quest/blog/?p=1895","disqusUrl":"https://ww2.kqed.org/quest/2009/03/30/producers-notes-worlds-most-powerful-microscope/","disqusTitle":"Producer's Notes: World's Most Powerful Microscope","videoEmbed":"https://www.youtube.com/watch?v=sCYX_XQgnSA","path":"/quest/1895/producers-notes-worlds-most-powerful-microscope","audioTrackLength":null,"parsedContent":[{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003cp>Today QUEST takes you behind the scenes to see the most powerful microscope in the world, which happens to be in our very own backyard in Berkeley. This transmission electron microscope lives at the \u003ca href=\"http://ncem.lbl.gov/\">National Center for Electron Microscopy\u003c/a>, at the Lawrence Berkeley National Lab. The microscope can produce images of things that are the size of half an atom of hydrogen. And hydrogen has the smallest atoms of any element – so that's pretty small. \u003c/p>\n\u003cp>The microscope is so big that it was hauled into the Center on a crane. It's housed in its own room, which is insulated to maintain an ideal temperature, and it's mounted on springs to isolate it from vibrations that make images blurry. \u003c/p>\n\u003cp>The \u003ca href=\"http://ncem.lbl.gov/TEAM-project/index.html\">TEAM 0.5\u003c/a>, as the microscope is called, excels at producing clear images of atoms sitting side by side. This makes it very useful for the scientists who investigate the properties of the materials that we use to build everyday objects like computers and airplanes. In fact, the images they produce with the microscope may one day help build stronger, lighter airplanes, and smaller, faster computers. \u003c/p>\n\u003cp>\u003c/p>\u003c/div>","attributes":{"named":{},"numeric":[]}},{"type":"component","content":"","name":"ad","attributes":{"named":{"label":"fullwidth"},"numeric":["fullwidth"]}},{"type":"contentString","content":"\u003cdiv class=\"post-body\">\u003cp>\u003c/p>\u003cp>\u003c/p>\n\u003c/div>\u003c/p>","attributes":{"named":{},"numeric":[]}}],"link":"/quest/1895/producers-notes-worlds-most-powerful-microscope","authors":["6186"],"categories":["quest_8","quest_3422","quest_3233"],"tags":["quest_247","quest_308","quest_959","quest_1624","quest_1815","quest_1816","quest_2893"],"label":"quest"}},"programsReducer":{"possible":{"id":"possible","title":"Possible","info":"Possible is hosted by entrepreneur Reid Hoffman and writer Aria Finger. 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You can also visit the MindShift website for episodes and supplemental blog posts or tweet us \u003ca href=\"https://twitter.com/MindShiftKQED\">@MindShiftKQED\u003c/a> or visit us at \u003ca href=\"/mindshift\">MindShift.KQED.org\u003c/a>","imageSrc":"https://cdn.kqed.org/wp-content/uploads/2024/04/Mindshift-Podcast-Tile-703x703-1.jpg","imageAlt":"KQED MindShift: How We Will Learn","officialWebsiteLink":"/mindshift/","meta":{"site":"news","source":"kqed","order":"2"},"link":"/podcasts/mindshift","subscribe":{"apple":"https://podcasts.apple.com/us/podcast/mindshift-podcast/id1078765985","google":"https://podcasts.google.com/feed/aHR0cHM6Ly9mZWVkcy5tZWdhcGhvbmUuZm0vS1FJTkM1NzY0NjAwNDI5","npr":"https://www.npr.org/podcasts/464615685/mind-shift-podcast","stitcher":"https://www.stitcher.com/podcast/kqed/stories-teachers-share","spotify":"https://open.spotify.com/show/0MxSpNYZKNprFLCl7eEtyx"}},"morning-edition":{"id":"morning-edition","title":"Morning Edition","info":"\u003cem>Morning Edition\u003c/em> takes listeners around the country and the world with multi-faceted stories and commentaries every weekday. 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On Our Watch brings listeners into the rooms where officers are questioned and witnesses are interrogated to find out who this system is really protecting. 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